Figure 4.

Effects of STMN1 knockdown on cell growth and apoptosis. (A) Cells were transfected with Non-silencing shRNA as a negative control or STMN1shRNA. Cell proliferation was measured by CCK-8. Data are expressed as percentage change (Means ± S.D.) compared with controls and represent six independent experiments. (P < 0.05 vs Non-silencing shRNA, one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparion). (B) Apoptosis was assessed by TUNEL assay, the amount of DNA fragmentation (apoptosis) was assessed by TUNEL assay for esophageal cancer cells before and after silencing stathmin gene expression utilizing STMN1 shRNA. There was no significant difference between controls and non-silencing shRNA (scrambled sequences). Experiments were performed in triplicate. (C) Cell-cycle distributions of control shRNA infected cells and (D) STMN1 shRNA infected cells as measured by flow cytometry.