Figure 1.

Enhanced RC-Seq

(A) Multiplexed Illumina libraries are hybridized to liquid-phase sequence capture probes targeting the 5′ and 3′ ends of recently active human retrotransposons (Table S1).

(B) Paired-end 150-mer sequencing of ∼220 nt inserts enables “contig” assembly of each read pair into a single read.

(C) Assembled reads with a 5′ or 3′ section of an active retrotransposon at one end (highlighted in red) are retained. The opposite end is then aligned to the reference genome, indicating the position of known and novel insertions.