Table 2.
Real-GP®, -GN®, and -CAN® real-time PCR assay results for discriminating the Gram-positive and -negative bacteria and Candida species in 115 clinical isolates
| Culture identification | No. of samples (n) |
Real-time PCR TaqMan assay |
||
|---|---|---|---|---|
| GP/GN or Candida | Ranged C T value | Mean C T value | ||
|
Staphylococcus aureus |
12 |
GP |
22.44-26.65 |
24.47 |
|
Staphylococcus spp. (CoNS) |
8 |
GP |
19.46-28.71 |
21.5 |
|
Streptococcus spp. |
5 |
GP |
17.35-30.46 |
24.25 |
|
Enterococcus faecalis |
4 |
GP |
25.2-27.3 |
26.37 |
|
Enterococcus faecium |
10 |
GP |
21.3-31.6 |
26.58 |
|
Enterococcus mundtii |
1 |
GP |
27.85 |
27.85 |
|
Corynebacterium spp. |
1 |
GP |
24.51 |
24.51 |
|
Escherichia coli |
16 |
GN |
12.68-30.65 |
23.26 |
|
Klebsiella pneumoniae |
13 |
GN |
15.48-26.08 |
20.73 |
|
Pseudomonas aeruginosa |
13 |
GN |
15.23-19.96 |
18.11 |
|
Acinetobacter baumannii |
11 |
GN |
18.09-24.65 |
21.04 |
|
Enterobacter asburiae |
2 |
GN |
15.79 |
15.79 |
|
Enterocobacter cloacae |
1 |
GN |
15.41 |
15.41 |
|
Moraxella catarrhalis |
1 |
GN |
33.54 |
33.54 |
|
Serratia marcescens |
1 |
GN |
21.64 |
21.64 |
|
Providencia rettgeri |
1 |
GN |
24.3 |
24.30 |
|
Morganella morganii |
1 |
GN |
20.6 |
20.60 |
|
Proteus mirabilis |
1 |
GN |
24.88 |
24.88 |
|
Aeromonas spp. |
1 |
GN |
25.97 |
25.97 |
|
Citrobacter fruendii |
2 |
GN |
17.11-18.01 |
17.56 |
|
Candida albicans |
5 |
CAN |
17.61-29.56 |
23.9 |
|
Candida parapsilosis |
3 |
CAN |
24.73-30.95 |
27.58 |
|
Candida tropicalis |
1 |
CAN |
26.62 |
26.62 |
|
Candida glabrata |
1 |
CAN |
17.68 |
17.68 |
| Total | 115 | |||