Figure 1.

A High Spatial and Temporal Resolution View of Genome Replication

(A) Replication profiles for chromosome 14. The six S phase time points (15, 20, 25, 30, 35, and 40 min) are relative to release from the cdc7-1 zero time point. Each data point represents the extent of DNA replication in a 1 kb window. Circles on the x axis represent the location of replication origins.

(B) Median replication time, Trep, derived from the time-course data shown in (A).

(C) Flow cytometry data for the time-course experiment (see also Figure S1).

(D) Quantification of the flow cytometry data that were used to normalize the deep sequencing data presented in (A).

(E) The two temporally separate stages of replication origin function, licensing and activation, that the mathematical model captures.

(F) Representative origin activation probability curves for an early activating origin (ARS606) and a late activating origin (ARS1412; see also Table S1).

(G) Median origin activation time correlates with the width of the activation distribution (see also Figure S2).

(H) Model fit (continuous line) to experimental replication-time-course data (points) for three representative time points.