Figure 5.

Inactivation of Replication Origins Results in Redistribution of Termination Events

(A) Replication profile for chromosome 6 and regions of chromosomes 7 and 10 (as described for Figure 1A).

(B and C) Comparisons of equivalent time points from the wild-type (black data) and origin mutant (gray data) are shown for each chromosomal region. Time points were selected to ensure equivalent levels of genome-wide DNA synthesis; time points are 15 and 20 min (wild-type) and 20 and 25 min (origin mutant) in (B) and (C), respectively. Vertical arrows indicate the inferred location of the highest probability termination sites.

(D) The inferred distribution of replication termination sites for the wild-type (black) and the origin mutant (gray). Data displayed as in Figure 4A. A red cross marks origins that were inactivated in the mutant strain (see also Figures S4 and S5).