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. 2013 Aug 20;7(6):1069–1082. doi: 10.1016/j.molonc.2013.07.011

Figure 4.

Figure 4

CD117+CD11blo LCs express early hematopoietic progenitor markers and lower levels of myeloid cell markers. LCs were collected from the spleens of leukemic mice and stained with cell surface markers as indicated. The phenotypes of LCs were analyzed by flow cytometry. To distinguish LCs from normal hematopoietic cells (both recipient and support BM cells are CD45.1+), cells were first gated on CD45.2+ (for MLL‐AF9 leukemia) or GFP+/YFP+ (for HoxA9/Meis1 leukemia) and then gated on CD117+ for further analysis (A). The percentages of CD117+CD11blo populations in p27+/+ and p27−/− MA9‐LCs as well as in p27+/+ and p27−/− H/M‐LCs were summarized (B). CD117+ LCs (the cells inside the red gate of figure A) were analyzed for expression of HSPC markers (C) and mature myeloid or B lymphocyte markers (D). Cells in the blue gate are CD117+CD11blo populations in all figures. Data shown in C and D are from p27+/+ MA9‐LCs. Similar data were obtained from p27−/− MA9‐LCs, p27+/+ H/M‐LCs and p27−/− H/M‐LCs (data not shown).