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. 2013 Aug 20;7(6):1069–1082. doi: 10.1016/j.molonc.2013.07.011

Figure 7.

Figure 7

CD117+CD11blo LCs are relatively more drug resistant than are CD117+CD11bhi LCs. Populations of CD117+CD11blo and CD117+CD11bhi LCs were isolated from the spleens of p27+/+ MA9, p27−/− MA9, p27+/+ H/M and p27−/− H/M leukemic mice by FACS. Cells were incubated in 96‐well plates in 4‐cytokine culture medium with indicated dosages of cytosine arabinoside (Ara‐C) or doxorubicin (DNR). Cells were seeded at a density of 2 × 104 or 1 × 103 per well for cell death or colony‐forming analysis, respectively. Cell death was determined after 16 h of treatment by Annexin‐V staining and analyzed by flow‐cytometry (A & B). The remaining colony‐forming cells in each well after 6 h of Ara‐C or doxorubicin treatment were examined by suspension culture in MethoCult® GF M3434 (C & D). ** indicates significant difference compared to other groups (p < 0.01).