Figure 4.

RNA Interference of TbPPL2 Triggers the Focal Accumulation of DNA Signaling and Repair Proteins

(A–C) Immunofluorescent detection of γH2A in TbPPL2, TbPriL, and TbPPL1 RNAi cell lines, grown in the presence or absence of tetracycline (1 μg/ml for the time indicated). Representative images including phase-contrast and DAPI-stained DNA are shown. The proportion of cells with nuclear foci were counted (n = 200) and error bars represent one standard deviation. Scale bar is 5 μm.

(D and E) Immunofluorescent detection of Rad51 in TbPPL2 and TbPriL RNAi cell lines. Other details as above.