Figure 6.

TbPPL2^Myc Accumulates into DNA Repair Foci

TbPPL2^Myc cells were grown in the presence or absence of 0.0003% MMS for 24 hr.

(A) Western blot analysis with anti-cMyc and cell lysates prepared from MMS-treated cells. The Coomassie-stained gel serves as a loading control.

(B) Immunofluorescence analysis of MMS-treated cells with anti-cMyc; the proportion of cells with TbPPL2^Myc staining was determined. n = 200; error bars, standard deviation.

(C) Representative images of cells scored in (B). Scale bar is 5 μm.

(D) Immunofluorescence analysis of MMS-treated cells with anti-cMyc (for PPL2) and anti-γH2A. Scale bar is 5 μm.