Figure 3.

Delta Waves, Spindles, and Ripples Are Desynchronized in MAM Animals

(A) Coherence between motor and visual cortical EEG during NREM sleep (bandwidth 0.2 Hz, 10 s moving window, 4 tapers) is significant in the 0.3–3 Hz frequency range in SHAM animals (black, n = 8, p < 0.05) and significantly reduced in the MAM animals (blue, n = 8, p < 0.01 versus SHAM), indicating impaired anterior-posterior delta wave coordination. (B) Motor delta wave (dotted line shows average delta waveform)-triggered spindle power shows similar patterns at anterior sites in SHAM and MAM animals (upper graph) but is severely disrupted at posterior cortical sites (lower graph) in MAM animals. Note the characteristic occurrence of spindles on the rising phase of delta waves in SHAM animals. (C) PrL spindle-triggered averaging of CA1 ripple power (from LFP, 10 ms bin width) shows that ripples consistently precede spindles in SHAM animals (black, n = 6) but that this timing relationship is abolished in MAM animals (blue, n = 5; Bonferroni corrected pairwise comparison ^∗p < 0.05). (D) Alignment of average PrL spindle waveforms (upper graph) with ripple band RMS activity shows fine timescale, spindle-frequency modulation of CA1 ripples in SHAM animals (middle graph) and its attenuation in MAM animals (lower graph). All data shown as mean ± SEM. See also Figure S3.