Figure 1.

Effect of SBL on clonogenic potential of malignant mesothelioma cells and non-malignant mesothelial MeT-5A cells. (A) Cells were precultured for 24 h, then treated with increasing doses of SBL (0.2, 2, 5, 10 and 20 μM) for 48 h. After treatment, cells were washed with medium and plated in a 6-well plate (MESO-1, 500; MESO-4 and H28, 1,000; MeT-5A, 3,000 cells/well, respectively). After 12 days, the colonies were fixed with 2% paraformaldehyde, and stained with 0.1% crystal violet. (B) Assays were done in triplicate, and the average of colony counts is presented.