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. 2013 Nov 28;44(2):377–384. doi: 10.3892/ijo.2013.2192

Figure 3.

Figure 3.

Figure 3.

Figure 3.

Combinatorial treatment with SBL and TRAIL shows synergistic cytotoxity. (A) Cytotoxic effect of combination of SBL and TRAIL in H28 cells. Cells were treated with SBL (5 μM) and/or TRAIL (1, 1.5 and 2 ng/ml) in combination for 24 h. The viability was determined by WST-1 assay. Data are expressed as the mean ± SD from three independent experiments in triplicate. *P<0.05 versus SBL alone. (B) Binding of Annexin V in combination-treated cells. H28 or MeT-5A cells were treated with SBL (5 μM), etoposide (50 μM) and/or TRAIL (2 ng/ml) for 24 h. The percentage of Annexin V-positive cells was determined using flow cytometric analysis. Data are expressed as the mean ± SD of three independent experiments. *P<0.02 versus TRAIL alone. (C) Caspase activation in combination-treated H28 cells. Cells were treated with SBL (5 μM) and/or TRAIL (2 ng/ml) for 24 h. The activation of caspase-8, -9 or -3 was determined by western blotting. β-actin was used as a standard to ensure equivalent loading of cell extracts.