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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Nat Methods. 2013 Oct 6;10(11):1122–1126. doi: 10.1038/nmeth.2687

Fig. 3. Instant SIM reveals ER dynamics at 100 Hz.

Fig. 3

A: First image in 200 timepoint series, showing ER labeled with GFP-Sec61A within MRL-TR transformed human lung fibroblasts. Data were acquired at the coverslip surface. Scalebar: 10 μm. b: Higher magnification view of the large white rectangle in a. White arrows mark growth of an ER tubule, blue arrows indicate remodeling of an ER tubule. Scalebar: 5 μm. c: Higher magnification view of the small white rectangle in a, indicating formation of a new tubule within 140 ms. Scale bar: 200 nm. See also Supplementary Video 10.

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