Fig. 5.

(a) Northern blot of RNA collected from E8B and CS2 parasites at different times after selection for adhesion to ICAM-1 and sequentially probed with sequences from var2csa, the 3′ end of exon 1 of It4_var44, and the conserved var exon 2. (b) Western blot probed with rabbit antiserum to the conserved exon 2 of PfEMP1; samples were CS2, CS2 selected on ICAM-1 and cultured until both var2csa and It4_var44 were transcribed (CI), E8B soon after selection on ICAM-1 when It4_var44 was transcribed (EI44+), and E8B selected on ICAM-1 and grown until transcription of It4_var44 had ceased (EI44-).