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Western blot analysis of TMEM16A protein constructs with anti-TMEM16A and anti-HA antibodies. A) Results show that anti-HA detects the protein when the HA epitope is placed after (M1X-HA, S16X-HA) and not before (HA-M1X) the stop codon. B) The anti-HA antibody does not react with the wild type TMEM16A protein.
