Figure 1. Multiple C-terminal isoforms of connexin 43 occur in human heart.
(A) Western blot of non-failing human left ventricle with monoclonal antibody against C×43 C-terminus. Intensity profiles of five separate hearts averaged and plotted in graph with peaks corresponding to major immunodetectable bands (arrows). Schematic: predicted methionine initiated polypeptides and their molecular weights. (B) Western blot of HaCaT cells transfected with cDNA encoding eGFP or C×43. (C) C×43 N- and C-terminus HA tag fusion proteins expressed in 293T cells. Western blot probed with monoclonal anti-HA antibody.