Figure 1.

Nucleoside Triphosphate Pyrophosphatase Activity of Purified Maf Proteins: Hydrolysis of Canonical and Modified Nucleotides

The graphs show specific activities of (A) YhdE, (B) BSU28050, (C) YOR111W, and (D) ASMTL-Maf against canonical and modified nucleotides (the left and right parts of the graph, respectively). The reaction mixtures contained 0.2 mM substrate, 2 mM Co²⁺, and 0.1–0.5 μg of enzyme. Results are means ± SD from at least two independent determinations. The molecular structures of positive Maf substrates are shown on the right side of the graph.

See also Figure S2 and Table S1.