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Site-Directed Mutagenesis of Maf Proteins: Nucleotide Pyrophosphatase Activity of Purified Mutant Proteins
(A–E) Mutant proteins shown are (A) YhdE, (B) BSU28050, (C) YOR111W, (D) ASMTL-Maf, and (E) YceF. The assays contained 0.3 mM UTP in (A) through (D) or 0.2 mM m7GTP (E), 2 mM Co2+ in (A) and (C), 5 mM Co2+ in (B) and (D), or 0.2 mM Co2+ (E) and 0.03–0.5 μg of protein. Results are means ± SD from at least two independent determinations.
See also Figures S1 and S5.
