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. 2004 Mar 19;6:35–54. doi: 10.1251/bpo71

Fig. 1. The anti-association activity of IF3.

Fig. 1

The extent of 70S initiation complex formation was monitored as a function of time by light scattering after rapid mixing in the stopped-flow instrument of a volume containing 30S subunits, mRNA, GTP and initiation factors as indicated with a volume containing 50S subunits. Time traces obtained with no initiation factors added to the 30S subunits (Panel A), with only IF3 added (Panel B), with IF1 and IF2 added (Panel C) and with IF1, IF2, IF3 and [3H]fMet-tRNAfMet added (Panel D).