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. 2004 Mar 19;6:35–54. doi: 10.1251/bpo71

Fig. 4. The effects of buffer composition on the rate of 70S initiation complex formation.

Fig. 4

The extent of 70S complex formation was monitored as a function of time by light scattering after rapid mixing of mixture A containing dissociated 70S ribosomes together with IF1, IF3 and mRNA with mixture B containing IF2:GTP together with fMet-tRNA. Complex formation in polymix buffer (PM) with 3 mM of free Mg2+ (Panel A), in PM buffer with 7 mM of free Mg2+ (Panel B) and in PM buffer with 3 mM free Mg2+ plus 10 mM of PEP (Panel C).