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. 2014 Jan 22;9(1):e85749. doi: 10.1371/journal.pone.0085749

Figure 5. Developmental changes in sizes of MC3T3 cells seeded in collagen constructs.

Figure 5

(A) –(D) Fluorescence staining of MC3T3 cells embedded in collagen constructs in control and osteogenic media on day 1 and day 21 (actin cytoskeleton is stained green, nuclei are stained blue). (E) Estimated diameter of cells from SUSI analysis over time in culture. Scale bar  = 50 µm. Best viewed in color.