Figure 6. HS is localized mainly to the basement membrane in both resting and inflamed human dermal microvascular blood endothelial cells (HDBECs).

Immunostaining for HS (red), actin (green) and nuclei (blue) of resting or IL1-β- stimulated HDBEC monolayers grown for 16–18 hours on fibronectin. The mean fluorescence intensity values shown are the mean±SEM of ten fields of view from three independent experiments. Images were taken with a Delta-Vision microscope at 60X magnification. Scale bar represents 15 µm. N.S. not significant. ***P<0.0001 by Student's t test.