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. 2014 Jan 22;9(1):e85980. doi: 10.1371/journal.pone.0085980

Figure 3. Downregulation of CADM1 in human lung mast cells reduced the assembly of filamentous actin and tyrosine phosphorylation.

Figure 3

A. HLMCs from various donors were transduced with SP4, control shRNA (LucSh) or CADM1 shRNA (Shm) viral particles, followed by fluorescent staining of surface CADM1 and F-actin. Surface CADM1 expression is shown in histograms. B. CADM1 in the SP4 and Shm groups was expressed as percentages of the control LucSh group and quantified as percentage and log-transformed percentage. (n = 5 for LucSh and SP4, n = 4 for Shm). These data were presented in Fig. 1 in [19]. C. HLMCs with modulated CADM1 were also co-stained for F-actin. The data are shown in the graph. Correlation between surface CADM1 and F-actin in LucSh- or Shm-transduced HLMCs is shown in a scatter plot with correlation parameters. E. Western blotting of protein extracts from LucSh-, SP4- and Shm-transduced HLMCs from D682 and D674, developed with Abs shown on the right. The protein bands were quantified and the data are shown in the graph.