Skip to main content
. 2014 Jan 22;9(1):e85980. doi: 10.1371/journal.pone.0085980

Figure 5. CADM1 downregulation in HMC-1 cells increased the length of cortical actin filaments.

Figure 5

LucSh-, SP4- and Shm-transduced HMC-1 cells from an experiment shown in Fig. 2C and 3A , stained for F-actin and CADM1, were examined by confocal laser scanning microscopy. The same optical section of the cell surface (equivalent to the side of a crumpet) is shown for each protein. F-actin and CADM1 are shown in false colours. The right column shows light-transmission images. Bar is 5 µm. B. The maximal length of actin filaments was calculated as the means of 4 longest distances between crossed filaments for each examined HMC-1 cell, then the data for n = (10–11) cells for each group were analysed. ** P<0.01; *** P<0.001. C. Graphs are shown for the thresholded Manders’s coefficients M1 for colocalisation of F-actin with CADM1 and M2 for colocalisation of CADM1 with F-actin in the SP4, LucSh and Shm-groups of cells (n = 7, n = 7 and n = 6 cells with two surfaces each, respectively). * P<0.05, ** P<0.01; *** P<0.001.