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. Author manuscript; available in PMC: 2015 Jan 15.
Published in final edited form as: Methods. 2013 Jul 22;65(2):165–174. doi: 10.1016/j.ymeth.2013.07.022

Table 3.

A comparison of commonly used PTP assays using 5 nM LYP.

Substrate Method Readout [substrate] S/B
Peptide 2 Fluorescence λex= 360 nm,
λem = 455 nm		 10 µM 10
Peptide 2a Fluorescence λex = 280 nm,
λem = 305 nm		 500 µM 2.0
Peptide 2a Malachite green Absorbance at
620 nm		 500 µM 6.0
MUP Fluorescence λex = 360 nm,
λem = 455 nm		 500 µM 6.5
DiFMUP Fluorescence λex = 360 nm,
λem = 455 nm		 0.50 µM 25