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Kinetic parameters for TCPTP-mediated hydrolysis of several peptide substrates
| Peptide # | Peptide sequence | kcat (s−1) | Km (mM) | kcat/Km (M−1s−1) |
|---|---|---|---|---|
| 3 | Ac-DIDE-pCAP-LAA-NH2 | 92 ± 5 | 0.32 ± 0.02 | 290,000 |
| 4 | Ac-FnGA-pCAP-QLEE-NH2 | 160 ± 30 | 0.27 ± 0.05 | 600,000 |
| 5 | Ac-VFDQ-pCAP-HESP-NH2 | 11.5 ± 0.5 | 0.28 ± 0.03 | 41,000 |
| 6 | Ac-DPHR-pCAP-VWKR-NH2 | NR | NR | NR |
