Table 5.
Kinetic parameters for PTPH1 and LYP-mediated hydrolysis of peptides based on TCR ζ-chain ITAMs
| Peptide | KM (µM) [95% CI] | kcat (s−1) [95% CI] | kcat/Km (s−1mM−1) | # of Experiments |
|
|---|---|---|---|---|---|
| PTPH1 | |||||
| 7; ITAM B pCAP/pY | 4.44 [3.88–5.08] | 0.77 [0.74–0.81] | 173.4 | 5 | |
| 8; ITAM B pY/pCAP | 4.26 [3.76–4.81] | 1.05 [1.01–1.09] | 246.5 | 5 | |
| 9; ITAM B pCAP/Y | 9.49 [8.68–10.34] | 2.16 [2.09–2.22] | 227.3 | 6 | |
| 10; ITAM B Y/pCAP | 8.64 [7.92–9.41] | 2.02 [1.96–2.07] | 233.1 | 6 | |
| 11; ITAM C pCAP/pY | 14.41 [10.90–17.91] | 0.33 [0.31–0.35] | 22.8 | 3 | |
| 12; ITAM C pY/pCAP | 19.09 [14.98–23.20] | 0.69 [0.65–0.72] | 36.0 | 3 | |
| 13; ITAM C pCAP/Y | 13.70 [10.36–17.04] | 1.12 [1.06–1.18] | 81.7 | 3 | |
| 14; ITAM C Y/pCAP | 25.13 [20.88–29.37] | 1.45 [1.38–1.52] | 57.7 | 3 | |
| LYP | |||||
| 7; ITAM B pCAP/pY | 1.77 [1.38–2.24] | 0.22 [0.20–0.24] | 125.4 | 4 | |
| 8; ITAM B pY/pCAP | 1.87 [1.36–2.49] | 0.36 [0.32–0.39] | 191.4 | 4 | |
| 9; ITAM B pCAP/Y | 6.81 [6.20–7.49] | 0.78 [0.76–0.81] | 114.9 | 3 | |
| 10; ITAM B Y/pCAP | 6.09 [5.26–7.02] | 0.76 [0.72–0.79] | 124.2 | 3 | |