. Author manuscript; available in PMC: 2015 Jan 15.

Published in final edited form as: Methods. 2013 Jul 22;65(2):165–174. doi: 10.1016/j.ymeth.2013.07.022

Table 6.

Kinetic parameters for VHR and MKP-5-mediated hydrolysis of peptides based on ERK1/2 activation loop

Peptide	K_M (µM) [95% CI]	k_cat (s⁻¹) [95% CI]	k_cat/K_m (s⁻¹mM⁻¹)	# of Experiments
VHR
15; ERK1/2 pT/pCAP	48.9 [36.6–61.2]	0.26 [0.23–0.28]	5.22	3
16; ERK1/2 T/pCAP	47.6 [33.1–62.0]	0.28 [0.25–0.31]	5.88	3
MKP-5
15; ERK1/2 pT/pCAP	25.8 [20.3–31.2]	0.24 [0.22–0.25]	9.19	3
16; ERK1/2 T/pCAP	15.5 [13.7–17.4]	0.16 [0.16–0.17]	10.63	3