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. 2014 Jan 22;9(1):e85874. doi: 10.1371/journal.pone.0085874

Figure 1. Dopamine reduces fat content of wild type animals.

Figure 1

(A–D) Animals exposed to increasing concentrations of dopamine exhibit reduced fat stores as visualized by vital dyes Nile Red and BODIPY-labeled fatty acids, fixative-based dyes Oil-Red-O and Sudan Black and CARS microscopy. (A) Representative images and (C) quantitation of fluorescence intensities. Black and gray bars represent Nile Red and BODIPY respectively. (B) Illustrative images and (D) quantitation of stain intensities. Black and white bars represent Oil-Red-O and Sudan Black respectively. (E) Representative images and (F) quantification of CARS signal intensities. (C–D) Data are showed as percentage of control (“No dopamine”) average ± SEM. (n = 12 to 15 animals per condition for Nile Red and BODIPY. n = 5 to 8 animals per condition for Oil-Red-O and Sudan Black). (F) Data are showed as absolute intensity ± SD. (n = 8 to 10 worms per condition). * p<0.05, ** p<0.01, *** p<0.001 compared to “No dopamine” animals.