Figure 2.

Experimental procedure for major steps involved in RL-SAGE library construction. A, Ditag PCR products (136 bp) were resolved on a 12% (w/v) polyacrylamide gel. B, PCR products (136 bp) were digested with NlaIII, and the ditag's (40 bp) band was purified from a 16% (w/v) polyacrylamide gel. C, Concatemers from self-ligated ditags were partially digested and resolved on a 6% (w/v) polyacrylamide gel. D, PCR inserts of RL-SAGE clones were separated on a 1.5% (w/v) agarose gel. E1-3, RL-SAGE concatemer clones were sequenced from rice, maize, and blast fungus libraries, and NlaIII sites separating two ditags are shown in bold.