Figure 4. Single nucleosome analysis of H4-PA-GFP. (A) Displacement (movement) histograms of single nucleosomes in interphase DM cells for 30 ms (left), 60 ms (center), and 90 ms (right; n = 20 000 in seven cells). The mean displacement ± standard error for the 30 ms time point was indicated. (B) Distribution of nucleosome displacements in the x-y plane for 30 ms (left), 60ms (center), and 90 ms (right). n = 3 000 in seven cells. Standard deviations (SD_x and SD_y) were shown. In panels (A) and (B), the originally calculated displacement was in two dimensions. To obtain their 3D values, the two-dimensional values of displacement were multiplied by $\sqrt{1.5}$ ($\sqrt{(6Dt/4Dt)}$). (C) Displacement histograms of single EGFP on a glass surface for 30 ms (left), 60 ms (center) and 90 ms (right; n = 1 300). The mean ± standard error for the 30 ms time point was indicated. (D) Distribution of EGFP displacements in the x-y plane for 30 ms (left), 60ms (center), and 90 ms (right; n = 1 300). Standard deviations (SD_x and SD_y) were shown. The insets are 3-fold magnified images of the distributions.