Figure 3. p24- and KF-specific antibody responses after intranasal immunization. BALB/c mice were immunized intranasally with 5 μg p24, 5 μg p24 plus 5 μg KF, 10 μg KF-p24 or 10 μg KFD-p24 3D at 0, 4th, and 8th weeks. (A and C) Time-course of KF-specific or p24-specific IgG titers in serum. (B and D) KF-specific or p24-specific IgA titers in serum at 6th and 10th weeks. (E) p24-specific IgA titers in mucosal sites including saliva, vaginal lavage fluid, and lung and small intestine at 10th week. (F) Ratios of p24-specific IgA titers in mucosal sites to serum IgG titers at 10th week. Data are presented as the means ± SEM from one experiment that was repeated three times (n = 6 per group).