Table 1. Oligonucleotide primers for generation of KF-p24 and KFD-p24 3D.
Plasmid | Restricted site | Sequence (5′-3′) |
---|---|---|
pKF-p24 | NcoI | GCG CCA TGGCAC AAG TCA TTA ATA CC |
EcoRI | GCG AAT TCA CCC TGC AGC AGA GAC AGA AC | |
EcoRI | GCC GAA TTC CCT ATA GTG CAG AAC CTC CAG | |
XhoI | GGA CTC GAG CAA AAC TCT TGC TTT ATG GCC | |
pKFN3 | NcoI | GGC GTC CAT GGC ACA AGT CAT TAA TAC |
BamHI | TAC TAG GAT CCG TCG TGC TAG CTC CAG ATG TGT GAG TTG TGT CAC CAA GTG GAG TAT CAA GGC CAA GAG TTT TAG |
|
pKFD-p24 3D | NheI | ATC GAG CTA GCA CAC CTC TTG GTG ATA CTA CAC ACA CAT CAG GAC CTA TAG TGC AGA ACC TCC A |
BamHI | TAT GCG GAT CCG TCG TCA AAA CTC TTG CTT TAT GGC |