Table III.
Present calls on the Barley1 GeneChip probe array when hybridized with labeled cRNA from barley first seedling leaf after inoculation with powdery mildew
Barley Accession (Feature) | Average No. of Present callsa | % of Total | Total Present Calls per Genotypeb | Present Calls Unique to a Specific Genotypeb |
---|---|---|---|---|
C.I. 16137 (Mla1c) | 13,343d ± 566 | 58.4e ± 2.5 | 11,322 | 243 |
C.I. 16151 (Mla6) | 13,025 ± 496 | 57.1 ± 2.2 | 11,269 | 244 |
C.I. 16155 (Mla13) | 13,518 ± 479 | 59.2 ± 2.1 | 11,703 | 438 |
Derived from the MAS 5.0 report file
Present in all six replications per genotype. There were 10,491 present calls in common among all three genotypes (18 hybridizations)
C.I. is the standard abbreviation for Cereal Introduction. Mla1, Mla6, and Mla13 are alleles conferring specific resistance to the powdery mildew fungus. Ten to 20 seedlings for each species were harvested and pooled for RNA preparation, labeling, and hybridization. Data were taken from six independent biological replications
Average ± sd of all present calls in each replication based on six independent RNA isolations. Seedlings harvested directly after inoculation with powdery mildew
Calculated as a percentage of baseline barley leaf probe sets that exceed the probe pair threshold (present calls)