Table 2. Iqg1, Cyk3 and Inn1 localization at the division site of large budded binucleate cells.

Strain genotypes	Minutes after α-factor release	Budded cells %	Binucleate cells %	Iqg1 at bud neck % ± SEM
IQG1-GFP	150	66.7	29.2	31.8 ± 1.5
GAL1-DMA2 IQG1-GFP	150	75.7	40.0	43.4 ± 1.8
				Cyk3 at bud neck % ± SEM
CYK3-GFP	120	86.3	66.4	22.2 ± 1.2
GAL1-DMA2 CYK3-GFP	120	88.3	69.3	2.8 ± 0.9
				Inn1 at bud neck % ± SEM
INN1-GFP	165	63.2	53.2	19.9 ± 1.1
GAL1-DMA2 INN1-GFP	165	67.4	47.3	19.8 ± 1.2
GAL1-DMA2 INN1-GFP hof1∆	165	82.9	61.3	1.0 ± 0.8

The percentages are the mean of the values obtained at the indicated time points in triplicates of the experiments described in Figure 3 (Iqg1) and Figure 4 (Cyk3 and Inn1). About 100 large budded binucleated cells were scored for protein localization in each cell culture for each experiment ± standard mean errors.