Figure 2. Romidepsin causes histone acetylation and a decrease in c-myc levels in Burkitt lymphoma cell lines.(A)Cells were treated with 25 ng/ml romidpesin (DP) for 24 h after which protein was extracted, proteins were separeated by PAGE, and transferred to nitrocellulose membranes. The membranes were subsequently probed for PARP, cleaved PARP, acetylated histone H3, c-myc and GAPDH. Results from one of two independent experiments are shown.(B)Cells were treated as in (A), after which c-myc staining was examined by immunohistohemistry as outlined in the methods section. Magnification, 40×.(C)Cells were treated with the noted concentrations of romidpesin (DP) after which RNA was isolated and PCR analysis was performed for MYC. MYC levels were normalized to rRNA levels. Results from 3 analyses of 2 independent treatments are shown.