Figure 5. Effect of HDI treatment on HCT-116 cells lacking Bak, Bax or both.(A)Whole-cell lysates were extracted from parental HCT-116 cells, or cells lacking Bak (BAK^−/−), Bax (BAX^−/−), or both Bak and Bax (DKO). HUT78 cells also served as a positive control for Bak and Bax. Proteins were separated by PAGE and were transferred to nitrocellulose membranes. The membranes were subsequently probed for Bak or Bax. GAPDH served as a loading control. Results from one of two experiments are shown.(B)HCT-116 cells were treated with the indicated concentrations of HDIs for 48 h, after which cells were incubated with annexin V antibody and propidium iodide. The percent annexin-positive cells were calculated and results from at least 3 independent experiments are shown. P < 0.0001 for all HDI treatments compared with control for HCT-116, BAK^−/−, and BAX^−/− cells; P < 0.0135 for all HDI treatments compared with control for DKO cells.