Table 1. Effective dose of methacholine (mg/kg) required to induce a 250% increase of airway resistance.

Group	Mean ± SD ED250
Control naive mice	0.234±0.043 *
OVA_Vehicle	0.089±0.031
OVA_ ASM-024 0.3 mg/kga	0.111±0.027
OVA_ ASM-024 1.5 mg/kga	0.145±0.032*
OVA_ ASM-024 3.0 mg/kga	0.128±0.037*
OVA_ ASM-024 3.0 mg/kgb	0.159±0.043*

^aInhalation, ^b Intranasal

^*p<0.05 compared to vehicle-treated OVA-sensitized mice.

ASM-024 administered by intranasal or inhalation routes immediately prior to the methacholine challenge to OVA-sensitized mice attenuated airway hyper-responsiveness.