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. 2014 Jan 22;9(1):e86269. doi: 10.1371/journal.pone.0086269

Figure 6. Endogenous IAA content and auxin distribution in the root tip were altered under glycerol treatment.

Figure 6

(A) Free IAA content in 6-day-old wild-type seedlings grown on 0.5×MS media plus 1% sucrose with or without 1 mM glycerol. The data are presented as the mean ± SE (n = 4). Asterisks (*) represent significant differences (p<0.05) by Student’s t-test. (B–E) DR5pro:GUS staining in 6-day-old seedlings grown on 0.5×MS medium containing various concentrations of glycerol (0 [B], 250 µM G [C], 1 mM G [D] and 5 mM G [E]). Photographs are representative of at least five stained plants. Bar = 100 µm. (F–I) DR5pro:GUS staining in seedlings grown on 0.5×MS medium containing various concentrations of glycerol and/or NPA (untreated control [F], 1 mM glycerol [G], 2 µM NPA [H] or 1 mM glycerol and 2 µM NPA [I]). DR5pro:GUS plants were grown on 0.5×MS medium for 4 days and subsequently transferred to medium with various additives for another 2 days of growth. The seedlings were then sampled for β-glucuronidase (GUS) staining. Bar = 100 µm.