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. 2014 Jan 22;9(1):e86269. doi: 10.1371/journal.pone.0086269

Figure 7. The effect of auxin transport inhibitor NPA on root development in Arabidopsis seedlings.

Figure 7

(A) Growth performance of wild-type seedlings under glycerol and NPA treatments. Wild-type seedlings were grown on 0.5×MS medium for 5 days and subsequently transferred to media containing 1 mM glycerol with or without 2 µM NPA for 3 days. Black dots indicated the starting growth positions of the PR tip after shift. Bar = 1 cm. (B) Primary root (PR) extension lengths and (C) Lateral root primordia (LRP) at Stages A and D in wild-type plants grown under the conditions as described in (A) were recorded. The data are presented as the mean ± SE (n = 36). (D) Comparison of wild-type and OE #16 plants under glycerol and NPA treatments. Wild-type and OE #16 seeds were sown directly onto media containing 0 mM glycerol, 1 mM glycerol and 2 µM NPA or 1 mM glycerol and 2 µM NPA for 7 days; the root lengths of the plants exposed to each treatment were then measured (n≥27). Different letters indicate significant differences (p<0.05).