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. 2014 Jan 22;9(1):e86342. doi: 10.1371/journal.pone.0086342

Figure 1. ERRγ induces CREBH gene.

Figure 1

(A,B), AML12 cells were infected with Ad-GFP or Ad-ERRγ for 24 hr. (A),Total RNA was isolated for qRT-PCR analyses to quantify CREBH mRNA levels using CREBH primers. (B), Western blot analyses shows CREBH full length (CREBH-F), and CREBH active form (CREBH-N) expression. (C), AML12 cells were infected with Ad-GFP or Ad-ERRα for 24 hr. Western blot analyses shows CREBH-N expression. (D,E), Ad-GFP or Ad-ERRγ were injected via tail vein into male C57BL/6J mice (n = 5 per group). Following completion of the experiments, mice were sacrificed, and liver tissues were obtained for (D), qRT-PCR analyses to quantify CREBH mRNA levels, and (E), western blot analyses of CREBH-N expression. (F,G), HepG2 cells were infected with Ad-GFP or Ad-ERRγ for 24 hr. (F),Total RNA was isolated for qRT-PCR analyses to quantify CREBH mRNA levels using CREBH primers. (G), Western blot analyses shows CREBH-N expression. Data are representative of three independently performed experiments and shown as mean±SD; *, P<0.05 using Student’s t-test.