Figure 2. Reduced minus oxidized difference absorbance spectra of S. oneidensis MR-1 solubilized membranes.

Solubilized membranes in 20-HCl pH 7.6, 5% glycerol, 1% DDM were oxidized using 1 mM potassium ferricyanide and reduced by adding few grains of sodium dithionite. Membranes were isolated from strain MR-1 grown under microaerobic conditions (spectra A and B) or aerobic conditions (spectra C and D). Bacterial cells were harvested in the exponential (spectra A and C) or stationary phase (spectra B and D) of growth. The concentration of proteins was 4.8 mg.mL⁻¹. The spectra were recorded at room temperature. The vertical bar indicates the absorption scale.