Table 3. Growth parameters and TMPD-dependent oxidase activity of oxidase deletion mutants cultivated in aerobic conditions.
Strain | Relevant genotype | Doubling timea | Relative yieldb | TMPD oxidase activityc |
MR-1 | wild-type | 54.2+/−0.2 | 1.00 | 90+/−6 |
SLL01 | ΔccoN | 78.0+/−0.7 | 0.77 | 0 |
SLL02 | ΔcydA | 53.4+/−2.1 | 1.00 | 91+/−9 |
SLL03 | ΔcoxA | 59.0+/−1.1 | 0.96 | 97+/−6 |
SLL05 | ΔcydAΔcoxA | 56.5+/−2.7 | 0.97 | 101+/−9 |
SLL06 | ΔccoNΔcoxA | 79.9+/−1.2 | 0.78 | 0 |
Expressed in min.
Relative yield is defined as the highest optical density in early stationary phase in mutant strains cultures relative to that in the wild-type strain cultures.
Expressed in nmol O2.min−1.mg protein−1.
Data were obtained from triplicate separated experiments.