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. 2014 Jan 22;9(1):e86279. doi: 10.1371/journal.pone.0086279

Figure 2. Effect of phosphorylation mimicking protein modifications on the rate of Ca2+ dissociation from the Tn complex.

Figure 2

Panel A shows the time courses of the increase in IAANS fluorescence as Ca2+ was removed by EGTA from control Inline graphic, TnI T144E Inline graphic, TnI T144D Inline graphic and TnI T144A Inline graphic. Panel B shows the time courses of the increase in IAANS fluorescence as Ca2+ was removed by EGTA from control Inline graphic, TnI S43/45E Inline graphic, TnI S43/45D Inline graphic, TnI S43/45A Inline graphic, and TnI S43/45E/T144E Inline graphic. Panel C shows the time courses of the increase in IAANS fluorescence as Ca2+ was removed by EGTA from control Inline graphic, TnT T194E Inline graphic, S198E Inline graphic, T284E Inline graphic and TnT T203E Inline graphic. The data traces have been staggered and normalized for clarity.