Figure 3. Effect of phosphorylation mimicking protein modifications on the Ca2+ sensitivity of the thin filament.
Panel A shows the Ca2+ dependent increases in IAANS fluorescence for control 
(□), TnI T144E 
(○), TnI T144D 
(▵) and TnI T144A 
(•) as a function of pCa. Panel B shows the Ca2+ dependent increases in IAANS fluorescence for control 
(□), TnI S43/45E 
(▽), TnI S43/45D 
(▪), TnI S43/45A 
(★), and TnI S43/45E/T144E 
(☆) as a function of pCa. Panel C shows the Ca2+ dependent increases in IAANS fluorescence for control 
(□), TnT T194E 
(
), S198E 
(♦), T284E 
(◊) and TnT T203E 
(▾) as a function of pCa. The data sets were normalized individually for each mutant.