Table 1. Effect of Phosphorylation Mimicking TnI and TnT Mutants on the Ca²⁺ Binding Properties of the Tn complex.

Protein	Tn Ca2+ Kd(µM)	Tn nH	Tn Ca2+ Koff (/s)
control	0.89±0.02	0.88±0.02	41.3±0.2
TnI T144E	0.90±0.09	0.85±0.02	40±1
TnI T144D	0.99±0.06	0.96±0.04	49±1*
TnI T144A	1.60±0.05*	0.96±0.07	50±1*
TnI S43/45E	1.4±0.1*	1.04±0.06	62±1*
TnI S43/45D	1.8±0.2*	0.91±0.04	63±1*
TnI S43/45A	1.4±0.1*	1.01±0.07	60±2*
TnI S43/45E/T144E	1.6±0.1*	1.13±0.06*	66±1*
TnT T194E	0.78±0.04	1.08±0.03*	40.6±0.4
TnT S198E	1.03±0.09	0.90±0.03	42.1±0.9
TnT T284E	0.84±0.09	1.02±0.04	40±1
TnT T203E	0.86±0.05	0.93±0.06	42.0±0.5

^*significantly different from their respective control values (p<0.05).