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Functional complementation of a yeast allantoinase mutant. The dal1 mutant was transformed with the empty vector (pYES2), with pYES2 carrying AtALN, or with pYES2 carrying RpALN. Three cell dilutions (1:10, 1:100, and 1:1,000) were plated on yeast minimal medium containing 0.1% (w/v) ALN as sole nitrogen sources and were incubated for 5 d at room temperature.
