Figure 6.
Differential expression of black locust allantoinase during the seed germination and seedling growth. A, Northern-blot analyses of RpALN in black locust seedlings. RpALN mRNA is differentially expressed in seedling tissues. According to the number of DAG, total RNA (10 μg) from the indicated tissues was electrophoresed and blotted. The RNA was then probed with [α32P]dCTP-labeled RpALN cDNA. Each bottom panel presents the corresponding agarose gel stained with ethidium bromide, showing ribosomal RNAs. B, Enzyme assay of allantoinase of seedling tissues. Total proteins were extracted from three tissues of 4- or 6-d-old or seedlings: cotyledon (black bar), hypocotyl (white bar), and root (hatched bar). Results represent means of two independent experiments.