Figure 7.

RpALN gene expression in trunk wood of black locust. A, Antisense northern-blot analysis of RpALN mRNA in seedling (SD), bark/cambial zone (BC), sapwood (SW), and transition zone (TZ) by antisense northern-blot analysis. Histon H 3.2 transcript levels were indicated as a control. Each sample lane contained an equal amount of 200 ng of aRNAs. B, Northern-blot analysis of RpALN mRNA using three different seasons of trunk wood. RpALN mRNA is differentially expressed in bark/cambial zones according to spring (SP), summer (SM), and fall (FL). Total RNA (10 μg) from the indicated season samples was separated and blotted, and the mRNA was then detected with [α³²P]dCTP-labeled RpALN cDNA. Bottom panel presents the corresponding agarose gel stained with ethidium bromide, showing ribosomal RNAs.