Effect of affinin on auxin-regulated gene expression. A, Twelve hours of GUS staining of DR5:uidA primary roots grown for 7 d in medium without auxin; B, under 10-8 m 2,4-D; C, treatments of 2.8 × 10-5 m affinin; C, 1.2 × 10-4 m affinin. E, Twelve hours of GUS staining of BA3:uidA primary roots grown for 7 d in medium without auxin, under 10-8 m 2,4-D (F), and treatments of 2.8 × 10-5 m affinin (G) and 1.2 × 10-4 m affinin (H). Photographs are representative individuals of at least 20 plants stained. Scale bar = 200 μm.